Preventive intervention with Agaricus blazei murill polysaccharide exerts anti-tumor immune effect on intraperitoneal metastasis colorectal cancer

Authors: Yongzhao Xu et al.

Journal: International Journal of Biological Macromolecules (2024)

Research Areas: Cancer Reserarch

Cell Lines: Mouse colorectal cancer cell (MC38)

Summary: Colorectal cancer has a high mortality rate and the prognosis of existing treatments is poor. Agaricus blazei murill (ABM) and its polysaccharide (ABM polysaccharide, ABMP) have the potential for immunomodulation and anti-tumor effects, but the immunomodulatory mechanism of ABMP in colorectal cancer is unclear. Subcutaneous and intraperitoneal tumor-bearing mouse models were constructed to simulate primary and metastatic colorectal cancer, so as to explore the inhibitory effect of ABMP on tumors and its influence on the fatty acid metabolism pattern in the tumor microenvironment (TME). In this study, the authors used the Halo counter HD-4 Cell Counter (Hiscore Inc, China) to regularly count MC38 - N4 target cells (red fluorescence) and MC38 - EGFP non-target cells (green fluorescence). This counting method can help monitor the proliferation and viability of the cultured cell line MC38 and ensure the influence of experimental conditions on cell growth. The research results show that ABMP inhibits the growth of intraperitoneal tumors, which may be related to the tumor stage. It exerts its anti-tumor effect by altering the fatty acid metabolism in the TME, increasing the content of ω-3 PUFA, and regulating the immune response.

Keywords: Agaricus blazei murill, ABMP, Immunoregulation, Peritoneal carcinomatosis, Raman, Tumor microenvironment, MCR-ALS